A Comprehensive Practical Guide to RNA Sequencing

RNA sequencing (RNAseq) is a powerful and increasingly popular tool that is used to investigate a variety of biological questions across the tree of life. However, while commercially available solutions have made the bench work associated with RNAseq easier than ever, the planning and analysis of an RNAseq experiment require considerable bioinformatics knowledge. In this course, students will learn how to design and analyze both single cell and bulk RNAseq experiments with topics including: quality control, mapping, read counting, identification of differentially expressed genes, gene set enrichment analysis, clustering and annotation, pseudotime, and advanced topics. Computer lab workshops will be incorporated to supplement lecture material and allow students to work on their own data.