Molecular and cellular analysis of human cancer cell lines

This laboratory course will use cultured mammalian cells to study whether drug treatment can restore function to mutant versions of the tumor suppressor p53. Students will perform a variety of cellular and molecular techniques, including western blot and immunofluorescence, to test certain abilities of their mutant in the presence and absence of the drug. The project will culminate with student-designed experiments testing a functional aspect of p53 and presentation of the results for both expert and lay audiences. Strongly suggested prerequisites: BIO 45, BIO 82, 83, 86. Enrollment very limited; application required. Apply by 2/10/23 at: https://docs.google.com/forms/d/e/1FAIpQLSeIZAf2oAqwu8y9RMca4BjPTo8coCHl2asCk24kHMvGxPNlfg/viewform?usp=sf_link